Mispa Lume - Top Ranked RT-LAMP Analyzer

Mispa Lume is a very small molecular diagnostics machine that can run 16 samples simultaneously at a quicker TAT for real-time target gene identification utilizing RT-LAMP reaction.

What is LAMP Technology:

LAMP is the short form for Loop-mediated isothermal amplification, a gene amplification assay for detecting the target gene. LAMP is faster than traditional PCR at analyzing genetic material. Reverse-transcription LAMP (RT-LAMP) amplifies RNA gene targets and has been used to find the COVID-19 virus. RT-LAMP with crude RNA samples can be used for quick testing at the point of care because it can use patient samples directly. It is crucial to select a diagnostic technique that is simpler and quicker than RT-PCR, depending on the circumstance. Both RT-LAMP and LAMP use four to 6 primers that recognize the target DNA. A strand-displacing DNA polymerase starts the process, and 2 of the primers form loop structures that make it easier for the next amplification round.

Specialties of an RT-LAMP Analyzer

RT-LAMP requires a constant temperature range from 60-72°C. InstaLume RT-LAMP analyzer is capable of maintaining a constant temperature and real-time fluorescence detection that enables real-time gene amplification monitoring. The InstaLume analyzer can run 1 to 16 samples at a time. The results can be obtained regarding Ct (Cycle threshold) values. One of the advantages of this technology is that the consumables require only 2-8*C, while RT PCR machine consumables are to be stored at -20*C during shelf life and transportation. Again, the analytical process is very simple, two steps, and any junior technician with little training can operate the system efficiently. Other technologies require well-trained technicians owing to the complexity of the testing process and technology.

Comparison of RT-LAMP with other technologies for the Detection of SARS-CoV-2

RT-LAMP, as discussed, is a simple, sensitive, quick, and cost-effective method that can find pathogens early. The reagents storage is between 2-8*C, and better performance is reported from established lab validations. InstaLume and SARS-Cov-2 are suitable reagents for very less TAT, and you get the results in 35 minutes post-sampling.

Mispa Lume gives accurate results with 100% specificity, 99.3% accuracy, and 98.7% sensitivity for Covid-19 confirmatory test. LAMP machine is available with affordable investment in smaller labs. Any smaller labs with less turnover also can use this machine with limited test numbers at a time.